Dr. Deorosan specializes in
in vitro methods of tissue engineering related to adult stem cell culture and collagen-based, three-dimensional cell scaffolds. He has extensive experience performing metabolic analysis and imaging analysis of cells.
Dr. Deorosan’s doctoral research at Purdue University focused on using two-dimensional and three-dimensional cell culture, chemical assays, and fluorescence imaging to evaluate the changing metabolic characteristics of bone marrow-derived stem cells as the cells were exposed to different chemical environments. Additionally, his doctoral research involved the in vitro simulation of central nervous system trauma to understand differences in post-injury viability between pluripotent cells and neural cells. His experience with biomaterials extends to the extraction of collagen from animal tissue, and the evaluation of biodegradable polymers. Additionally, he has been involved in the preparation of and the animal implantation of collagen-based composites to be used for the treatment of critical bone defects.
Dr. Deorosan has also worked in micromanufacturing clean rooms during the design and construction of microfludics devices to be used for the real-time analysis of cell responses to chemical stimuli. His experience with microfludics extends to photolithography, soft lithography, and finite element analysis pertaining to the fluid dynamics of the resulting final channel arrangement.
Deorosan B. In vitro metabolic assessment of bone marrow-derived stem cells and its application to central nervous system trauma. Doctoral Dissertation, Purdue University, 2010.
Deorosan B, Nauman EA. The role of glucose, serum, and three-dimensional cell culture on the metabolism of bone marrow-derived mesenchymal stem cells. Stem Cells International 2011; Epub: April 5, 2011.
Selected Presentations
Deorosan B. Characterizing the metabolic state of adult stem cells in both two-dimensional and three-dimensional environments. Purdue University Biomedical Engineering Summer Symposium, 2007.
Caldorera ME, Deorosan BA, Haley VP, Hiers EA, Moore AL, McShane MJ. Self-contained system for observation of chemically stimulated cell cultures. 22nd Annual Houston Conference on Biomedical Engineering Research, Houston, TX, 2005.