Jessica F. Greene, Ph.D., DABT

Dr. Greene is a board-certified toxicologist with 14 years experience in the field of Toxicology. Her primary interest and expertise is in mechanism of toxicity and metabolic activation (or deactivation) of toxins. Recent work has focused on computational toxicology—specifically structure-activity relationships to obtain insight into potential toxicity of unknown compounds. She has experience evaluating the potential toxicity of both exogenous and endogenous compounds in food and biomaterials. In addition, she has experience with US and EU regulatory systems, particularly in support of EU summary dossiers in compliance with EU Directive 91/414/EEC.

Prior work has involved examination of the toxicity and bioactivation of oxidized long-chain fatty acids, in vitro and in vivo experimentation with phase I and phase II detoxication enzymes, particularly epoxide hydrolase and cytochrome p450 1A1/2, as well as work with recombinant systems.

Work at Exponent has included state-of –the-art and mechanistic examinations of industrial chemicals and pollutants, including 2,3,7,8-TCDD, beryllium, and asbestos, among others. Dr. Greene has worked with the biomaterials industry on evaluation of potential toxicity of materials with long-term residence in the body. She has also worked with various food industry clients on exposure assessment and potential toxicity of chemicals in food-contact materials and in food and beverages, including lead and acrylamide. Dr. Greene has prior and current work experience evaluating the potential toxicity of new and existing pesticides. Dr. Greene has experience in risk assessment, design and execution of toxicological studies, method development, data collection and analysis, and exposure reconstruction.

Brorby GP, Sheehan PJ, Berman DW, Greene JF, Holm SE. Re-creation of historical chrysotile-containing joint compounds. Inhalation Toxicol 2008; 20:1043–1053.

Greene JF, Hays S, Paustenbach DJ. A proposed reference dose (RfD) for dioxin based on a weight-of-evidence evaluation of the animal and human studies. J Toxicol Environ Health, Part B 2003; 6:115-159.

Paustenbach DJ, Madl AK, Greene JF. Identifying an appropriate occupational exposure limit (OEL) for beryllium: Data gaps and current research initiatives. Appl Occup Environ Hyg J 2001; 16(5):527-538.

Greene JF, Williamson KC, Newman JW, Morisseau C, Hammock BD. Metabolism of methyl leukotoxin: bioactivation and detoxification. Arch Biochem Biophys 2000; 376:420-432.

Greene JF, Newman JW, Williamson KC, Hammock BD. Structure-activity relationships of epoxy fatty acids to cytotoxicity. Chem Res Toxicol 2000; 13:217-226.

Greene JF, Zheng J, Grant DF, Hammock BD. Cytotoxicity and protein binding of 1,2-epoxynaphthalene: Application of a new system for bioactivation of environmental toxins. Toxicol Sci 2000; 53:352-360.

Morisseau C, Goodrow M, Dowdy D, Zheng J, Greene JF, Hammock BD. Ureas, carbamates, and related compounds are potent inhibitors of soluble epoxide hydrolases. Proc Natl Acad Sci 1999; 96(16):8849-8854.

Moghaddam M, Grant D., Cheek J, Greene J, Williamson K, Hammock B. Bioactivation of leukotoxins to their toxic diols by epoxide hydrolase. Nat Med 1997; 3(5):562-566.

Grant DF, Greene JF, Pinot F, Borhan B, Moghaddam MF, Hammock BD, McCutchen B, Ohkawa H, Luo G, Guenthner TM. Development of an in situ toxicity assay system using recombinant baculoviruses. Biochem Pharmacol 1996; 51:503-515.

Non-Peer Reviewed Publications

Greene, J. News and Events. J. Children’s Health 2004; 2(3/4).

Greene, J. News and Events. J. Children’s Health 2004; 2(2).

Greene, J. News and Events. J. Children’s Health 2004; 2(1).

Greene, J. News and Events. J. Children’s Health 2003; 1(4).

Greene, J. News and Events. J. Children’s Health 2003; 1(3).

Greene, J. News and Events. J. Children’s Health 2003; 1(2).

Greene, J. News and Events. J. Children’s Health 2003; 1(1)

Greene JF, Paustenbach D. A proposed reference dose for dioxin of 1-10 pg/kg-day: A weight-of-evidence evaluation of the human studies. Organohal Comp 2002; 59:123-126.

Warmerdam JM, Greene JF. Analysis of the sex ratios from Seveso and Ranch Hand cohorts using binomial probability. Organohal Comp 2002; 59: 391-393.

Finley B, Fehling K, Greene JF, Hays SM. The presence of Ah-receptor agonists in the diet: Implications for risk assessment and management. Organohal Comp 2000; 47:365-368.

Greene JF, Hammock BD. Toxicity of linoleic acid metabolites. pp. 471–477. In: Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury. Honn et al. (ed), Plenum Publishers, New York, NY, 1999.

Presentations

Sheehan P, Hicks J, Goswami E, Lau E, Greene J, Fedoruk MJ. Assessment of mechanics’ exposure to benzene in mineral spirit solvents during parts washing activities. Presented at the American Industrial Hygiene Conference and Expo, Chicago, IL, May 13–18, 2006.

Goswami E, Greene J, Sheehan P, Hicks J. Analysis of exposure to benzene in mineral spirit solvents during parts washing and degreasing operations. Presented at the Society of Toxicology Meeting, San Diego, CA, March 5–9, 2006.

Greene, J., G. Brorby, and D. Paustenbach. 2004. Reentry criteria for dioxin and dioxin-like compounds for building surfaces. Society of Toxicology 43rd Annual Meeting, Baltimore, MD, March 21–25, 2004.

Yost L, Greene J, Hays S, Kelsh M, Li A, Sheehan P. Derivation of a range of interim inhalation slope factors for TCE using physiologically based pharmacokinetic modeling. Presented at the Society of Toxicology 43rd Annual Meeting, Baltimore, MD, March 2004.

Greene J, Paustenbach D. A proposed reference dose for dioxin of 1-10 pg/kg-day: A weight of evidence evaluation of the human studies. Presentation at Dioxin 2002, Barcelona, 2002.

Greene J, Paustenbach D. A proposed reference dose for dioxin of 5-10 pg/kg-day: A weight of evidence evaluation of the human studies. Poster presentation at United States-Vietnam Scientific Conference on Human Health and Environmental Effects of Agent Orange/Dioxins, Hanoi, Vietnam, 2002.

Greene JF, Williams P, Avadhanam K, Paustenbach D. An alternative approach for toxicity testing of genetically modified foods. Poster presentation at the Society of Toxicology Annual Meeting, San Francisco, CA, 2001.

Greene JF. Beryllium: History and toxicology. Presentation at the Genetic and Environmental Toxicology Association of Northern California Annual Meeting, Emeryville, CA, 2000.

Greene JF, Newman JW, Williamson KC, Hammock BD. Structure-activity relationships of epoxy fatty acids. Poster presentation at the Society of Toxicology Annual Meeting, New Orleans, LA, 1999.

Greene JF, Hammock BD. Toxicity and metabolism of leukotoxin and leukotoxin diol. Presentation at the Center for Environmental Health Sciences Annual Retreat, Sacramento, CA, 1998.

Greene JF, Morisseau C, Hammock BD. Specific activity of new fatty acid substrates with soluble epoxide hydrolase. Poster presentation at the Society of Toxicology Annual Meeting, Seattle, WA, 1998.

Greene JF, Williamson KC, Hammock BD. Detoxification of leukotoxin and isoleukotoxin by conjugation with glutathione. Presentation at the NIEHS Symposium of Toxicology and Environmental Chemistry, Davis, CA, 1997.

Greene JF, Williamson KC, Hammock BD. Epoxy fatty acid ‘protoxins’ detoxified by glutathione conjugation. Presentation at the Eicosanoids and Other Bioactive Lipids in Cancer and Related Diseases Conference, La Jolla, CA, 1997.

Greene JF, Williamson KC, Moghaddam MF, Grant DF, Hammock BD. Epoxy fatty acid ‘protoxins’ bioactivated by soluble epoxide hydrolase. Poster presentation at the Superfund Basic Research Program Annual Meeting, Berkeley, CA, 1996.

Greene JF, Williamson KC, Moghaddam MF, Grant DF, Hammock BD. Metabolism of fatty acid epoxides to their toxic diols by epoxide hydrolase. Poster presentation at the 7th North American ISSX Meeting, San Diego, CA, 1996.

Greene JF, Grant DF, Hammock BD. Toxicity of 1-naphthol to P4501A1 and Lac Z expressing Sf-21 cells through use of the baculovirus system. Poster presentation at the NIEHS Symposium of Toxicology and Environmental Chemistry, Davis, CA, 1995.

• Research Assistant UC Davis Departments of Environmental Toxicology and Entomology, 1994–1999
• Teaching Assistant /Mentor Departments of Environmental Toxicology and Entomology UC Davis, 1995–1999
  

Analyzed and evaluated toxicological literature for GRAS submission for multiple products.

Reformulated historical construction materials for use in present-day experiments.

Retained and deposed as testifying expert in case involving food-contact chemicals.

Performed structure-activity analyses for multiple chemicals using computational toxicology (DEREK) and standard toxicological analysis.

Provided toxicological support for preparation of more than 10 EU summary dossiers.

Evaluated potential toxicity of long-term residency and degradation of bio-materials in the body.

Provided support in several potential food recall cases involving food contaminants.

Performed analysis of levels of nicotine and tobacco byproducts in the body after smoking different types of cigarettes.

Prepared experts, lines of questioning for opposing experts, literature review and safe-use determination review in Proposition 65 case involving metals in paint.

Retained as expert witness in case involving severity of eye irritancy of soaps and related cleaning products.

Provided support for a human health risk assessment of an abandoned mine site containing 60,000 yards of mine tailings and mixed overburden impacted by arsenic and other chemicals. Post closure assessment showed no human health risk.

Prepared human health and ecological risk assessment for phenol contamination in ponds at gold mining site in Tanzania.

Prepared human health risk assessment for dioxin and dioxin-like compounds in dust in buildings near the World Trade Center site.

Prepared state-of-the-art reports including literature review, analysis of toxicological data, analysis of air sampling data, review of regulatory involvement and time-lines regarding alleged acute and chronic health effects associated with occupational exposure to beryllium.

Wrote multiple opinion letters, prepared trial presentations, prepared expert for deposition and trial in multiple cases regarding occupational exposure to allegedly toxic chemicals, including beryllium, hydrochloric acid, methyl ethyl ketone, arsenic, and others.

Managed a $500,000 research project for a major industrial manufacturer, which includes analysis of hundreds of human tissue samples, assessment of validity of testing methodology and laboratory accuracy and coordination of expert panels to discuss these issues.

Reviewed the literature on and analyzed the non-cancer effects of dioxin in order to provide comments to regulatory agencies and publish peer-reviewed article.

Wrote a multi-tiered protocol for safety testing of genetically modified foods.

Managed an exposure reconstruction project regarding exposure of a sub-contractor to natural gas exhaust in order to provide litigation support. Designed sampling and analysis program for multiple chemical exposures.

Identified and wrote white sheets for over 70 biological, chemical and radiologic weapons that have the potential to be used for terrorism.