Elly Sinkala
Elly Sinkala, Ph.D.
Associate
Biomedical Engineering
  • Menlo Park

Dr. Sinkala is a biomedical engineer with expertise in applying microfabrication and microfluidics techniques to address unmet experimental needs. During her postdoctoral appointment at UC Berkeley, she led an interdisciplinary project with Stanford School of Medicine to develop a novel technique for multiplexed, quantitative protein analysis of individual cancer cells.

In her doctoral research, she developed an electrode calibration device for in vivo neurobiology studies. The device is a more accurate, cost-effective alternative to current systems and is currently used worldwide at over 20 institutions thus far. Dr. Sinkala also has worked on projects to design and develop devices that provide both precise chemical and oxygen gradients to characterize cell responses (cell proliferation, migration and chemotropism). Through these projects, Dr. Sinkala has gained in depth experience in rapid device prototyping (soft lithography), assay development, mammalian cell culture, and primary cell analysis. Her bioanalytical expertise includes Western blot, immunocytochemistry, and epifluorescent microscopy. She routinely uses Matlab and ImageJ for data analysis and AutoCAD for design. She has co-authored several successfully funded grants from both private and public agencies.

Dr. Sinkala has a B.E. in Biomedical Engineering from Vanderbilt University and a Ph.D. in Bioengineering from the University of Illinois at Chicago.

CREDENTIALS & PROFESSIONAL HONORS

  • Postdoctoral Fellow, Bioengineering, University of California, Berkeley, 2015
  • Ph.D., Bioengineering, University of Illinois, Chicago, 2013
  • B.E., Biomedical Engineering, Vanderbilt University, 2008
  • National Institutes of Health Innovators DSP Fellow, 2013–2015

    National Science Foundation/ Louis-Stokes Alliance Minority Program Doctorate Fellow, 2008–2010

Publications

Sinkala E, Herr AE. Single-cell western blotting. In: Single Cell Protein Analysis. Methods in Molecular Biology Series, Singh AK, Chandrasekaran A (eds), Springer, 2015.

Sinkala E, McCutcheon JE, Schimdt E, Schuck M, Roitman MF, Eddington DT. Electrode calibration with a microfluidic flow cell for fast scan cyclic voltammetry. Lab on a Chip 2012; 2(13):2403–2008.

Lo JF, Sinkala E, Eddington DT. Oxygen gradient for open well via microfluidic substrates. Lab on a Chip 2010; 10(18):2394–2401.

Sinkala E, Eddington DT. Oxygen sensitive microwells. Lab on a Chip 2010; 10(23):2394–2401.

Presentations

Duncombe TA, Kang CC, Sinkala E, Ward TM, Pegram MD, Herr AE. Single-cell targeted proteomics for deep profiling of human breast cancer signaling. 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), Gyeongju, Korea, October 25–29, 2015.

Sinkala E, Sollier E, Renier C, Che J, Jeffrey SS, Herr AE. Label-free concentration of viable breast cancer CTCs for single cell Western blotting. 106th Annual Meeting of the American Association for Cancer Research, Philadelphia, PA, April 18–22, 2015.

Sinkala E, Sollier E, Jeffrey SS, Herr AE. Microfluidic western-blotting of rare breast cancer cells. 18th International Conference of Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014), San Antonio, TX, October 26–30, 2014.

Sinkala E, Eddington DT. A microfluidic device for the automated delivery of chemicals to brain slices and other tissues. Biomedical Engineering Society (BMES), Atlanta, GA, October 24-27, 2012.

Sinkala E, McCutcheon JE, Roitman MF, Eddington DT. Electrode calibration with a microfluidic flow cell for fast scan cyclic voltammetry. Society for Lab Automation and Screening (SLAS), San Diego, CA, February 5–8, 2012.

Sinkala E, Eddington DT. Oxygen-sensitive microwells. Biomedical Engineering Society (BMES), Austin, TX, October 7–10, 2010.

Sinkala E, Eddington DT. Polystrene microwells for cellular isolation. Lab Automation, Palm Springs, CA, January 23–27, 2010.

Sinkala E, Eddington DT. Micropatterned thin polystyrene films for single cell culture. Biomedical Engineering Society, Pittsburgh, PA, October 7–10, 2009.

CREDENTIALS & PROFESSIONAL HONORS

  • Postdoctoral Fellow, Bioengineering, University of California, Berkeley, 2015
  • Ph.D., Bioengineering, University of Illinois, Chicago, 2013
  • B.E., Biomedical Engineering, Vanderbilt University, 2008
  • National Institutes of Health Innovators DSP Fellow, 2013–2015

    National Science Foundation/ Louis-Stokes Alliance Minority Program Doctorate Fellow, 2008–2010